Synonyms
KIAA0521 antibody, MGC15913 antibody, P114-RhoGEF antibody, ARHGEF18 antibody, SA-RhoGEF antibody, Rho-specific guanine nucleotide exchange factor p114 antibody, rho guanine nucleotide exchange factor 18 antibody, P114-RHO-GEF antibody, 114 kDa Rho-specific guanine nucleotide exchange factor antibody, septin-associated RhoGEF antibody, Rho/Rac guanine nucleotide exchange factor 18 antibody, p114RhoGEF antibody, Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
131 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 770 and 990 of ARHGEF18 (Uniprot ID# Q6ZSZ5)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18(107-10328) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10328 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.