Anti-ARHGEF3 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9NR81
Gene Id	50650
Gene Symbols	ARHGEF3
Protein Name / Synonyms	ARHGEF3 (Center)
Clonality	Polyclonal
Preparation	This ARHGEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human ARHGEF3.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Specificity

Predicted Reactivity

Mouse, Chicken, Monkey

Other Names

ARHGEF3; Rho guanine nucleotide exchange factor 3; Exchange factor found in platelets and leukemic and neuronal tissues

NCBI Accession #

NP_001122087.1;NP_001122088.1;NP_062455.1

Other Accession #

Q91X46; Q9N0A8; Q5ZLX4; NP_062455.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

59783

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	ARHGEF3 Antibody (Center) (Cat# 102-16588) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ARHGEF3 antibody detected the ARHGEF3 protein (arrow).
	ARHGEF3 Antibody (Center) (Cat# 102-16588) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGEF3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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