Anti-ARNT (Center V528) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P27540
Gene Id	405
Gene Symbols	ARNT
Protein Name / Synonyms	ARNT (Center V528)
Clonality	Polyclonal
Preparation	This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IF, Western Blotting
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1. A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants.

Specificity

Predicted Reactivity

Bovine

Other Names

ARNT; BHLHE2; Aryl hydrocarbon receptor nuclear translocator; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta

NCBI Accession #

NP_001184254.1;NP_001659.1;NP_848514.1

Other Accession #

Q9BE97; NP_848514.1; NP_001659.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

86636

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of A549 cell stained with hARNT-V528(Cat# 102-12423).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).hARNT immunoreactivity is localized to Nucleus significantly.

ARNT Antibody (V528) (Cat# 102-12423) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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