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Anti-ARPC2 Antibody
Catalog #: 107-10307
ARPC2 antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 10109 |
| Protein Name / Synonyms | ARPC2 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
ARC34 antibody, PNAS-139 antibody, p34-Arc antibody, ARPC2 antibody, ARP2/3 protein complex subunit 34 antibody, actin-related protein 2/3 complex subunit 2 antibody, arp2/3 complex 34 kDa subunit antibody, actin related protein 2/3 complex, subunit 2, 34kDa antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
34 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 14 and 300 of ARPC2 (Uniprot ID# O15144)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:5000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2(107-10307) antibody at 1:100 dilution.
ARPC2 antibody detects ARPC2 protein by Western blot analysis.
A. 50 µg mouse kidney lysate/extract
10 % SDS-PAGE
ARPC2 antibody (107-10307) dilution: 1:2000
A. 50 µg mouse kidney lysate/extract
10 % SDS-PAGE
ARPC2 antibody (107-10307) dilution: 1:2000
ARPC2 antibody detects ARPC2 protein by Western blot analysis.
A. 50 µg rat kidney lysate/extract
10 % SDS-PAGE
ARPC2 antibody (107-10307) dilution: 1:5000
A. 50 µg rat kidney lysate/extract
10 % SDS-PAGE
ARPC2 antibody (107-10307) dilution: 1:5000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2(107-10307) antibody at 1:200 dilution.
Sample(30 µg of whole cell lysate)
A:293T
B: HeLa S3
12% SDS PAGE
107-10307 diluted at 1:500
A:293T
B: HeLa S3
12% SDS PAGE
107-10307 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.