Anti-ARRB1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P49407
Gene Id	408
Gene Symbols	ARRB1
Protein Name / Synonyms	ARRB1 (C-term)
Clonality	Polyclonal
Preparation	This ARRB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-363 amino acids from the C-terminal region of human ARRB1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described, however, their exact functions are not known.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Rabbit

Other Names

ARRB1; ARR1; Beta-arrestin-1; Arrestin beta-1

NCBI Accession #

NP_004032.2;NP_064647.1

Other Accession #

P29066; Q95223; Q8BWG8; P17870; NP_004032.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

47066

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	ARRB1 Antibody (C-term) (Cat# 102-11348) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ARRB1 antibody detected the ARRB1 protein (arrow).
	ARRB1 Antibody (C-term) (Cat# 102-11348)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of ARRB1 Antibody (C-term)(Cat# 102-11348) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
	ARRB1 Antibody (C-term) (Cat# 102-11348) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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