Synonyms
DKFZp564L0862 antibody, FLJ20636 antibody, MGC4954 antibody, ASB9 antibody, ankyrin repeat and SOCS box-containing 9 antibody, ASB-9 antibody, ankyrin repeat and suppressor of cytokine signaling box protein 9 antibody, ankyrin repeat and SOCS box protein 9 antibody, ankyrin repeat and SOCS box containing 9 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
32 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 283 of ASB9 (Uniprot ID# Q96DX5)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ASB9(107-10674) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ASB9(107-10674) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A:HeLa S3
B:Hep G2
C:MOLT4
12% SDS PAGE
107-10674 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.