3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-ASL Antibody
Catalog #: 107-11084
ASL antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 435 |
| Protein Name / Synonyms | ASL |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
ASAL antibody, ASL antibody, arginosuccinase antibody, argininosuccinase antibody, argininosuccinate lyase antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 13 and 261 of ASL (Uniprot ID# P04424)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASL(107-11084) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ASL(107-11084) antibody at 1:500 dilution.
ASL antibody detects ASL protein by Western blot analysis.
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
ASL antibody (107-11084) dilution: 1:1000
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
ASL antibody (107-11084) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Argininosuccinate Lyase(107-11084) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
10% SDS PAGE
107-11084 diluted at 1:1000
A: Hela
B: Hep G2
10% SDS PAGE
107-11084 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.