Anti-ASPA (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P45381
Gene Id	443
Gene Symbols	ASPA
Protein Name / Synonyms	ASPA (N-term)
Clonality	Polyclonal
Preparation	This ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human ASPA.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.

Specificity

Predicted Reactivity

Bovine

Other Names

ASPA; ACY2; ASP; Aspartoacylase; Aminoacylase-2

NCBI Accession #

NP_000040.1;NP_001121557.1

Other Accession #

P46446

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

35735

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of ASPA Antibody (N-term) (Cat# 102-16976) in MCF7 cell line lysates (35ug/lane). ASPA (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of ASPA (arrow) using rabbit polyclonal ASPA Antibody (N-term) (Cat# 102-16976). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.
	Confocal immunofluorescent analysis of ASPA Antibody (N-term)(Cat# 102-16976) with MCF-7 cell followed by Alexa Fluor^® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).