Synonyms
ASPEP antibody, DAP antibody, DNPEP antibody, aspartyl aminopeptidase antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 225 of Aspartyl aminopeptidase (Uniprot ID# Q9ULA0)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Aspartyl aminopeptidase antibody [N1N3] detects Aspartyl aminopeptidase protein at cytoplasm on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
Aspartyl aminopeptidase antibody [N1N3] (107-10018) dilutio

Sample(30 µg of whole cell lysate)
A:H1299
7.5% SDS PAGE
107-10018 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.