Ataxin 3 antibody, 0.73 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4287 |
| Protein Name / Synonyms | Ataxin 3 |
| Concentration (lot specific) | 0.73 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Wnt-beta-Catenin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AT3 antibody, ATX3 antibody, JOS antibody, MJD antibody, MJD1 antibody, SCA3 antibody, ATXN3 antibody, spinocerebellar ataxia type 3 protein antibody, ataxin-3 antibody, ataxin 3 variant h antibody, olivopontocerebellar ataxia 3 antibody, ataxin 3 variant ref antibody, josephin antibody, ataxin 3 variant m antibody, Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) antibody, Machado-Joseph disease protein 1 antibody, ataxin 3 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
42 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 245 of Ataxin 3 (Uniprot ID# P54252)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Ataxin 3(107-11147) antibody at 1:500 dilution.
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.
293T mock transfected (-) or transfected with a construct expressing human Ataxin 3 (+).
10 % SDS-PAGE
Ataxin 3 antibody (107-11147) dilution: 1:5000
293T mock transfected (-) or transfected with a construct expressing human Ataxin 3 (+).
10 % SDS-PAGE
Ataxin 3 antibody (107-11147) dilution: 1:5000
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody (107-11147) diluted by 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.