Anti-ATF3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P18847
Gene Id	467
Gene Symbols	ATF3
Protein Name / Synonyms	ATF3 (C-term)
Clonality	Polyclonal
Preparation	This ATF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the C-terminal region of human ATF3.
Concentration (lot specific)	0.300 mg/ml
Recommended Applications	Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

ATF3; Cyclic AMP-dependent transcription factor ATF-3; Activating transcription factor 3

NCBI Accession #

NP_001025458.1;NP_001035709.1;NP_001193413.2;NP_001193415.1;NP_001193417.2;NP_001665.1

Other Accession #

P29596; Q60765; Q2KII1; NP_001025458.1; NP_001665.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

20576

Recommended Dilutions

WB: 1:1000; FC: 1:10-50

	ATF3 Antibody (C-term) (Cat# 102-13050) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ATF3 antibody detected the ATF3 protein (arrow).
	Western blot analysis of ATF3 (arrow) using rabbit polyclonal ATF3 Antibody (C-term) (Cat# 102-13050). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene.
	ATF3 Antibody (C-term) (Cat# 102-13050) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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