Anti-ATF4 (S245) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q96AQ3
Protein Name / Synonyms	ATF4 (S245)
Clonality	Polyclonal
Preparation	This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-252 amino acids from human ATF4.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.

Specificity

Other Names

Tax-responsive enhancer element B67; ATF4 protein

Other Accession #

P18848

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Recommended Dilutions

WB: 1:1000

	Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245) (Cat# 102-16728). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).
	Western blot analysis of ATF4 Antibody (S245) Pab (Cat# 102-16728) pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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