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Anti-ATF7 (N-term) Antibody
Catalog #: 102-18053
Rabbit Anti-Human ATF7 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P17544 |
| Gene Id | 11016 |
| Gene Symbols | ATF7 |
| Protein Name / Synonyms | ATF7 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ATF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human ATF7. |
| Concentration (lot specific) | 0.470 mg/ml |
| Recommended Applications | Western Blotting, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The cyclic AMP-dependent transcription factor ATF7 is a member of the bZIP family of proteins and is known to bind to the cAMP response element (CRE) as a homodimer.
Specificity
Other Names
ATF7; ATFA; Cyclic AMP-dependent transcription factor ATF-7; Activating transcription factor 7; Transcription factor ATF-A
NCBI Accession #
NP_001123532.1;NP_001193611.1;NP_001193612.1;NP_006847.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
52967
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; FC: 1:10-50
 | ATF7 Antibody (N-term) (Cat# 102-18053) western blot analysis in huamn placenta tissue lysates (35ug/lane).This demonstrates the ATF7 antibody detected the ATF7 protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with ATF7 Antibody (N-term)(Cat# 102-18053).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ATF7 immunoreactivity is localized to Nucleus and Cytoplasm significantly. |
 | ATF7 Antibody (N-term) (Cat# 102-18053) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.