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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | Q86TL0 |
| Gene Id | 84971 |
| Gene Symbols | ATG4D |
| Protein Name / Synonyms | ATG4D |
| Clonality | Monoclonal |
| Preparation | This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Specificity
Other Names
ATG4D; APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D
NCBI Accession #
NP_001268433.1;NP_116274.3
Other Accession #
NP_116274.3
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
52922
Recommended Dilutions
IF: 1:25; WB: 1:100-1000
 | Immunofluorescent analysis of U251 cells, using ATG4D Antibody(Cat# 102-10125). U251 cells(right) were treated with Chloroquine (50 µM,16h). 102-10125 was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue). |
 | ATG4D/MB10250 antibody (Cat# 102-10125) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ATG4D/MB10250 antibody detected the ATG4D/MB10250 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.