Anti-ATG4D (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q86TL0
Gene Id	84971
Gene Symbols	ATG4D
Protein Name / Synonyms	ATG4D (Center)
Clonality	Polyclonal
Preparation	This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human ATG4D.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Specificity

Predicted Reactivity

Pig

Other Names

ATG4D; APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D

NCBI Accession #

NP_001268433.1;NP_116274.3

Other Accession #

Q684M2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

52922

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-APG4D Pab (Cat# 102-13684) in mouse kidney tissue lysate. APG4D(arrow) was detected using the purified Pab.
	Western blot analysis of anti-hAPG4D-L235 Pab (Cat# 102-13684) in 293 cell line lysates transiently transfected with the APG4D gene (2ug/lane). hAPG4D-L235(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	APG4D Antibody (L235) (Cat# 102-13684) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APG4D antibody detected the APG4D protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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