Anti-ATG5 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9H1Y0
Gene Id	9474
Gene Symbols	ATG5
Protein Name / Synonyms	ATG5
Clonality	Polyclonal
Preparation	This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human ATG5.
Concentration (lot specific)	1.780 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Specificity

Other Names

ATG5; APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein

NCBI Accession #

NP_004840.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

32447

Recommended Dilutions

IF: 1:200; WB: 1:1000; IHC: 1:10-50

	Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-13692 ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
	ATG5 Antibody (Cat# 102-13692) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the ATG5 antibody detected the ATG5 protein (arrow).
	APG5 Antibody (Cat# 102-13692) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the APG5 antibody detected the APG5 protein (arrow).
	ATG5 Antibody (Cat# 102-13692)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATG5 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
	APG5 Antibody (Cat# 102-13692b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG5 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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