Anti-ATP synthase delta Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	513
Protein Name / Synonyms	ATP synthase delta
Concentration (lot specific)	0.66 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Energy Metabolism, Mitochondria Function
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ATP5D antibody, ATP synthase subunit delta, mitochondrial antibody, mitochondrial ATP synthase, delta subunit antibody, mitochondrial ATP synthase complex delta-subunit precusor antibody, F-ATPase delta subunit antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

17 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 168 of ATP synthase delta (Uniprot ID# P30049)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

ATP synthase delta antibody detects ATP5D protein at mitochondria on breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded breast carcinoma.
ATP synthase delta antibody (107-10266) dilution: 1:250.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10266 diluted at 1:1000

Sample(30 ug whole cell lysate)
A:Hep G2
B:MOLT4
C:Raji
12% SDS PAGE
107-10266 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products