Anti-ATP5B (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P06576
Gene Id	506
Gene Symbols	ATP5B
Protein Name / Synonyms	ATP5B (Center)
Clonality	Polyclonal
Preparation	This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.
Concentration (lot specific)	0.398 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

ATP5B; ATPMB; ATPSB; ATP synthase subunit beta, mitochondrial

NCBI Accession #

NP_001677.2

Other Accession #

P10719; P56480; P00829

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

56560

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of ATP5B Antibody (Center) Pab (Cat# 102-15255) pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab.
	Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center) (Cat# 102-15255). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2) .
	Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	ATP5B Antibody (Center) (Cat# 102-15255) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products