Anti-ATP5O (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P48047
Gene Id	539
Gene Symbols	ATP5O
Protein Name / Synonyms	ATP5O (N-term)
Clonality	Polyclonal
Preparation	This ATP5O antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-64 amino acids from the N-terminal region of human ATP5O.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.

Specificity

Predicted Reactivity

Bovine, Pig

Other Names

ATP5O; ATPO; ATP synthase subunit O, mitochondrial; Oligomycin sensitivity conferral protein

NCBI Accession #

NP_001688.1

Other Accession #

Q2EN81; P13621

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

23277

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of ATP5O Antibody (N-term) (Cat# 102-17919) in 293 cell line lysates (35ug/lane). ATP5O (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
	Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of ATP5O (arrow) using rabbit polyclonal ATP5O Antibody (N-term) (Cat# 102-17919). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATP5O gene.