Anti-ATP6V1B1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P15313
Gene Id	525
Gene Symbols	ATP6V1B1
Protein Name / Synonyms	ATP6V1B1 (Center)
Clonality	Polyclonal
Preparation	This ATP6V1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-310 amino acids from the Central region of human ATP6V1B1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken, Drosophila, C.Elegans

Other Names

ATP6V1B1; ATP6B1; VATB; VPP3; V-type proton ATPase subunit B, kidney isoform; Endomembrane proton pump 58 kDa subunit; Vacuolar proton pump subunit B 1

NCBI Accession #

NP_001683.2

Other Accession #

P31409; P49712; Q19626; P62815; P62814; P21281; P31408; P31407; NP_001683.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

56833

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	ATP6V1B1 Antibody (Center) (Cat# 102-11134) western blot analysis in WiDr,K562 cell line lysates (35ug/lane).This demonstrates the ATP6V1B1 antibody detected the ATP6V1B1 protein (arrow).
	ATP6V1B1 Antibody (Center) (Cat# 102-11134)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP6V1B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of ATP6V1B1 Antibody (Center)(Cat# 102-11134) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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