Synonyms
ATPB-3 antibody, CD298 antibody, FLJ29027 antibody, ATP1B3 antibody, sodium/potassium-transporting ATPase beta-3 chain antibody, sodium/potassium-dependent ATPase subunit beta-3 antibody, sodium/potassium-dependent ATPase beta-3 subunit antibody, "Na, K-ATPase beta-3 polypeptide antibody", sodium/potassium-transporting ATPase subunit beta-3 antibody, "ATPase, Na+/K+ transporting, beta 3 polypeptide antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
32 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 53 and 279 of ATPase beta3 (Na+/K+) (Uniprot ID# P54709)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using ATPase beta3 (Na+/K+)(107-11121) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
ATPase beta3(Na+/K+) antibody
107-11121 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.