3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Aurora-A (C-term) Antibody
Catalog #: 102-17060
Rabbit Anti-Human Aurora-A (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O14965 |
| Gene Id | 6790 |
| Gene Symbols | AURKA |
| Protein Name / Synonyms | Aurora-A (C-term) |
| Clonality | Polyclonal |
| Preparation | This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human Aurora-A. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy.
Specificity
Other Names
AURKA; AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A
NCBI Accession #
NP_003591.2;NP_940835.1;NP_940836.1;NP_940837.1;NP_940838.1;NP_940839.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
45809
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of anti-Aurora-A Pab (Cat# 102-17060) in A2058 cell line lysate.Aurora-A(arrow) was detected using the purified Pab. |
 | Western blot analysis of Aurora-A (arrow) using rabbit polyclonal Aurora-A Antibody (C-term) (Cat# 102-17060). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Aurora-A gene (Lane 2) (Origene Technologies). |
 | Confocal immunofluorescent analysis of Aurora-A Antibody (C-term)(Cat# 102-17060) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | Aurora-A Antibody (C-term) (Cat# 102-17060) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.