Anti-Aurora-C (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UQB9
Gene Id	6795
Gene Symbols	AURKC
Protein Name / Synonyms	Aurora-C (N-term)
Clonality	Polyclonal
Preparation	This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-38 amino acids from the N-terminal region of human Aurora-C.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.

Specificity

Other Names

AURKC; AIE2; AIK3; AIRK3; ARK3; STK13; Aurora kinase C; Aurora 3; Aurora/IPL1-related kinase 3; Aurora/IPL1/Eg2 protein 2; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase aurora-C

NCBI Accession #

NP_001015878.1;NP_001015879.1;NP_003151.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

35591

Recommended Dilutions

WB: 1:1000

The anti-Aurora C Pab (Cat# 102-17057) is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3), Flag-tagged Aurora C (lane 4), and in A375 cell lysate. In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data with Flagged Aurora is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).

Aurora Antibody (C) (Cat# 102-17057) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the Aurora antibody detected the Aurora protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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