Anti-Bak (BH3 Domain Specific) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q16611
Gene Id	578
Gene Symbols	BAK1
Protein Name / Synonyms	Bak (BH3 Domain Specific)
Clonality	Polyclonal
Preparation	This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-91 amino acids from human Bak.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Specificity

Other Names

BAK1; BAK; BCL2L7; CDN1; Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7

NCBI Accession #

NP_001179.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

23409

Recommended Dilutions

IHC: 1:50-100; WB: 1:1000

	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Bak Antibody (BH3) (Cat# 102-11828) western blot analysis in Jurkat,MDA-MB453,293 cell line lysates (35ug/lane).This demonstrates the Bak antibody detected the Bak protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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