Anti-BARON (N-term) Antibody

Product Description

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q92622
Gene Id	9711
Gene Symbols	KIAA0226
Protein Name / Synonyms	BARON (N-term)
Clonality	Polyclonal
Preparation	This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the N-terminal region of human BARON.
Concentration (lot specific)	0.360 mg/ml
Recommended Applications	Western Blotting, IF, IHC-P, FC
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Other Names

KIAA0226; Run domain Beclin-1 interacting and cystein-rich containing protein; Beclin-1 associated RUN domain containing protein

NCBI Accession #

NP_001139114.1;NP_055502.1

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

108622

Recommended Dilutions

WB: 1:1000; IF: 1:100; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of BARON antibody (N-term) (Cat# 102-13787) in MCF-7 cell line lysates (35ug/lane). BARON (arrow) was detected using the purified Pab.
	Fluorescent image of U251 cells stained with BARON (N-term) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-13787 BARON (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
	Formalin-fixed and paraffin-embedded human lymph with BARON Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of MDA-231 cells using BARON Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HUMAN

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Product Description