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Anti-Bax (BH3 Domain Specific) Antibody
Catalog #: 102-11833
Rabbit Anti-Human Bax (BH3 Domain Specific) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q07812 |
| Gene Id | 581 |
| Gene Symbols | BAX |
| Protein Name / Synonyms | Bax (BH3 Domain Specific) |
| Clonality | Polyclonal |
| Preparation | This Bax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-76 amino acids from human Bax. |
| Concentration (lot specific) | 1.600 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Specificity
Predicted Reactivity
Bovine
Other Names
BAX; BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4
NCBI Accession #
NP_004315.1;NP_620116.1;NP_620118.1;NP_620119.2
Other Accession #
O02703; Q07814
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
21184
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Bax Antibody(BH3)(Cat# 102-11833) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Bax antibody detected the Bax protein (arrow). |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Bax Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of HepG2 cells using Bax Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of Bax (arrow) using rabbit polyclonal Bax Antibody (BH3) (Cat# 102-11833). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bax gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.