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Anti-BBOX1 Antibody
Catalog #: 107-10367
BBOX1 antibody, 0.76 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 8424 |
| Protein Name / Synonyms | BBOX1 |
| Concentration (lot specific) | 0.76 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
BBH antibody, BBOX antibody, G-BBH antibody, gamma-BBH antibody, BBOX1 antibody, gamma-butyrobetaine hydroxylase antibody, gamma-butyrobetaine,2-oxoglutarate dioxygenase 1 antibody, gamma-butyrobetaine dioxygenase antibody, butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
45 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 293 of BBOX1 (Uniprot ID# O75936)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
BBOX1 antibody detects BBOX1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
BBOX1 antibody (107-10367) dilution: 1:500.
Sample: Paraffin-embedded Ca922 xenograft.
BBOX1 antibody (107-10367) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10367 diluted at 1:500
A: mouse liver
10% SDS PAGE
107-10367 diluted at 1:500
Immunofluorescence analysis of methanol-fixed A431, using BBOX1(107-10367) antibody at 1:500 dilution.
Sample(30 µg of whole cell lysate)
A:MOLT4
7.5% SDS PAGE
107-10367 diluted at 1:500
A:MOLT4
7.5% SDS PAGE
107-10367 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.