Synonyms
TOK-1 antibody, TOK1 antibody, BCCIP antibody, TOK-1alpha antibody, BRCA2 and Cip1/p21 interacting protein antibody, BRCA2 and CDKN1A-interacting protein antibody, BCCIPbeta antibody, TOK-1beta antibody, cdk inhibitor p21 binding protein antibody, protein TOK-1 antibody, BCCIPalpha antibody, p21- and CDK-associated protein 1 antibody, BRCA2 and CDKN1A interacting protein antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 181 and 273 of BCCIP (Uniprot ID# Q9P287)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

BCCIP antibody detects BCCIP protein at nucleus on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
BCCIP antibody (107-11054) dilution: 1:500.

BCCIP antibody detects BCCIP protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
BCCIP antibody (107-11054) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BCCIP (107-11054) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: A431
B: Hela
10% SDS PAGE
107-11054 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.