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Anti-bcl-2 Antibody, Biotinylated
Catalog #: RB-02-0024B-50
Rabbit anti-bcl-2 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NP_000624 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide corresponding to the N-terminal region of Bcl-2. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Immunogen Sequence | CMKYIHYKLSQRGYEWDAGD |
| Isotype | IgG2a |
| Recommended Applications | ELISA (recommended work dilution= 1:40000 (detects ?25 µg/ml) ), Western Blotting (recommended work dilution= 1: 5, 000), (see image)Western blot analysis of 100 ng Bcl-2 using purified rabbit anti-Bcl-2 primary antibody (1:5, 000 µg/ml). The blot was incubated with anti-rabbit HRP-conjugated IgG (1:10, 000). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Apoptosis |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
B-cell lymphoma 2 (Bcl-2) is the member of the Bcl-2 family of apoptosis regulator proteins encoded by the BCL2 gene. The two isoforms of Bcl-2, Isoform 1, also known as 1G5M, and Isoform 2, also known as 1G5O/1GJH, exhibit similar fold. However, results in the ability of these isoforms to bind to the BAD and BAK proteins, as well as in the structural topology and electrostatic potential of the binding groove, suggest differences in antiapoptotic activity for the two isoforms. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody was selected for its ability to specifically detect Bcl-2 from humans. There is no cross reactivity with Caspase-3, Caspase-8 or Livin.
References
- Tsujimoto, Y. et al., (1984). "Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation". Science 226 (4678): 109799.
- Cleary, ML. et al., (1986). "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation". Cell 47 (1): 1928.
Expiration:
12 months from the date of shipment when stored properly.