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Anti-Bcl-w Antibody
Catalog #: 130-10025
Rabbit Anti-Human Bcl-w Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | O88996 |
| Gene Id | 60434 |
| Gene Symbols | Bcl2l2 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Bcl-w. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000(at least detecting 12.5ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Apoptosis |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Bcl-w,which is a member of the BCL-2 protein family, form hetero- or homodimers and act as anti- and pro-apoptotic regulators. This protein has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons.Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat Bcl-w has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month, otherwise, Store at -20°C. Minimize freezing and thawing when use.
Related Products
Rabbit Anti-bcl-2 Antibody(Cat#RB-02-0024) Goat Anti-Human BCL-2 Binding Component 3 Antibody(Cat#DS-PB-00241) RayBio® Human Apoptosis Array 1 (2) (Cat# AAH-APO-1-2) Bcl-2 ELISA Kit(Cat#ELH-Bcl2-001)
References
- Gibson L, Holmgreen SP, Huang DC. (1996) bcl-w, a novel member of the bcl-2 family, promotes cell survival.Oncogene.13(4):665-75.
- Murphy B, Dunleavy M, Shinoda S, Schindler C.(2007) Bcl-w protects hippocampus during experimental status epilepticus. Am J Pathol. 171(4):1258-68.
Expiration:
12 months from the date of shipment when stored properly.