Bcl-X antibody, 0.95 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 598 |
| Protein Name / Synonyms | Bcl-X |
| Concentration (lot specific) | 0.95 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
BCL-XL/S antibody, BCL2L antibody, BCLX antibody, BCLXL antibody, BCLXS antibody, Bcl-X antibody, DKFZp781P2092 antibody, bcl-xL antibody, bcl-xS antibody, BCL2L1 antibody, apoptosis regulator Bcl-X antibody, bcl-2-like protein 1 antibody, protein phosphatase 1, regulatory subunit 52 antibody, BCL2-like 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
26 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 208 of Bcl-X (Uniprot ID# Q07817)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A549, using BCL-x(107-10543) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
107-10543 diluted at 1:1000
A: HCT116
12% SDS PAGE
107-10543 diluted at 1:1000
Bcl-X antibody detects BCL2L1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12 % SDS-PAGE
Bcl-X antibody (107-10543) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
12 % SDS-PAGE
Bcl-X antibody (107-10543) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1(107-10543) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
107-10543 diluted at 1:1000
A:NIH-3T3
12% SDS PAGE
107-10543 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.