Anti-BCL2L15 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	440603
Protein Name / Synonyms	BCL2L15
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Apoptosis
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

Bfk antibody, C1orf178 antibody, FLJ22588 antibody, BCL2L15 antibody, pro-apoptotic Bcl-2 protein antibody, bcl-2-like protein 15 antibody, bcl2-L-15 antibody, BCL2-like 15 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

18 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 163 of BCL2L15 (Uniprot ID# Q5TBC7)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

BCL2L15 antibody detects BCL2L15 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCL2L15 protein stained by BCL2L15 antibody (107-10941) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using BCL2L15(107-10941) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
107-10941 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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