Anti-BDNF (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P23560
Gene Id	627
Gene Symbols	BDNF
Protein Name / Synonyms	BDNF (C-term)
Clonality	Polyclonal
Preparation	This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Neuroscience
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this protein is reduced in both Alzheimer's and Huntington disease patients. This protein may play a role in the regulation of stress response and in the biology of mood disorders.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

BDNF; Brain-derived neurotrophic factor; Abrineurin

NCBI Accession #

NP_001137277.1;NP_001137278.1;NP_001137279.1;NP_001137280.1;NP_001137281.1;NP_001137282.1;NP_001137283.1;NP_001137284.1;NP_001137285.1;NP_001137286.1;NP_001137288.1;NP_001700.2;NP_733927.1;NP_733928.1

Other Accession #

P23363; P14082; P21237; Q95106

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

27818

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of BDNF Antibody (C-term)(Cat# 102-17740) in CEM cell line lysates (35ug/lane). BDNF (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain reacted with BDNF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of BDNF Antibody (C-term)(Cat# 102-17740) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	Flow cytometric analysis of CEM cells using BDNF Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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