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Anti-Beclin 1 Antibody
Catalog #: 102-12166
Rabbit Anti-Human Beclin 1 Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q14457 |
| Gene Id | 8678 |
| Gene Symbols | BECN1 |
| Protein Name / Synonyms | Beclin 1 |
| Clonality | Polyclonal |
| Preparation | This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-123 amino acids from human Beclin 1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM].
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Chicken, Pig, Xenopus
Other Names
BECN1; GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197
NCBI Accession #
NP_003757.1
Other Accession #
Q6GP52; Q91XJ1; Q4A1L5; O88597; Q5ZKS6; Q4A1L4; NP_003757.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
51896
Recommended Dilutions
IF: 1:100; WB: 1:1000
 | Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-12166 Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. |
 | Beclin 1 Antibody (Cat# 102-12166) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Beclin 1 antibody detected the Beclin 1 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.