Mouse Anti-Beta-Actin Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Accession Number | P60709 |
| Gene Id | 60 |
| Gene Symbols | ACTB |
| Protein Name / Synonyms | Beta-Actin |
| Clonality | Monoclonal |
| Preparation | This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IHC-P, FC, Western Blotting |
| Research Area | Cardiovascular Disease, Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Specificity
Predicted Reactivity
Rat, Bovine, Chicken, Pig, Monkey, Xenopus
Other Names
ACTB; Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed
NCBI Accession #
NP_001092.1
Other Accession #
A2BDB0; P63259; P63260; P63261; Q5ZMQ2; P63258; P60711; Q6QAQ1; P60710; Q4R561; P60706; P60712; P53505
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
41737
Recommended Dilutions
IHC: 1:10-50; IF: 1:10-50; WB: 1:1000
 | ACTB Antibody (102-10055)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of ACTB Antibody (Cat# 102-10055) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue). |
 | Western blot analysis of anti-ACTB Antibody (Cat# 102-10055) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.