Anti-beta Actin Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	60
Protein Name / Synonyms	beta Actin
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Cytoskeletal Reorganization
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

PS1TP5BP1 antibody, ACTB antibody, "actin, cytoplasmic 1 antibody", beta cytoskeletal actin antibody, PS1TP5-binding protein 1 antibody, "actin, beta antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

42 kDa

Immunogen

Synthetic peptide contain a sequence corresponding to a region within amino acids 309 and 358 of beta Actin

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin(107-10210) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Immunohistochemical analysis of paraffin-embedded Prostate, using beta Actin(107-10210) antibody(10 µg/ml).

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGE
107-10210 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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