Synonyms
MADD antibody, ETFB antibody, beta-ETF antibody, electron transfer flavoprotein subunit beta antibody, "electron transfer flavoprotein, beta polypeptide antibody", electron transfer flavoprotein beta-subunit antibody, electron transfer flavoprotein beta subunit antibody, "electron-transferring-flavoprotein, beta polypeptide antibody", "electron-transfer-flavoprotein, beta polypeptide antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
28 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 255 of Beta ETF (Uniprot ID# P38117)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-10821 diluted at 1:10000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-ETF(107-10821) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: MOLT4
12% SDS PAGE
107-10821 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.