beta Tubulin 4 antibody, 0.41 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 10382 |
| Protein Name / Synonyms | beta Tubulin 4 |
| Concentration (lot specific) | 0.41 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
TUBB5 antibody, beta-5 antibody, TUBB4 antibody, "tubulin, beta 4 class IVa antibody", tubulin beta-4 chain antibody, class IVa beta-tubulin antibody, "tubulin, beta, 5 antibody", tubulin beta-4A chain antibody, tubulin 5 beta antibody, "tubulin, beta 4 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
50 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 410 of beta Tubulin 4
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 4(107-10316) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-10316 diluted at 1:1000
A: NIH-3T3
10% SDS PAGE
107-10316 diluted at 1:1000
Sample(30 µg of whole cell lysate)
A:293T
7.5% SDS PAGE
107-10316 diluted at 1:1000
A:293T
7.5% SDS PAGE
107-10316 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.