Anti-beta Tubulin Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	203068
Protein Name / Synonyms	beta Tubulin
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Cytoskeletal Reorganization
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

M40 antibody, MGC117247 antibody, MGC16435 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, TUBB antibody, beta1-tubulin antibody, beta-4 tubulin antibody, tubulin beta-1 chain antibody, tubulin beta chain antibody, tubulin beta polypeptide antibody, "tubulin, beta polypeptide antibody", class I beta-tubulin antibody, beta Ib tubulin antibody, beta 5-tubulin antibody, tubulin beta-5 chain antibody, "tubulin, beta antibody"

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

50 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 429 of beta Tubulin

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(107-10252) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin(107-10252) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-10252 diluted at 1:5000

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
107-10252 diluted at 1:10000

beta Tubulin antibody detects beta Tubulin protein at cytoplasm on breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded breast carcinoma.
beta Tubulin antibody (107-10252) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
10% SDS PAGE
107-10252 diluted at 1:3000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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