Rabbit anti-Betacellulin Antibody Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | AAH11618 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000) , Western Blotting (recommended work dilution= 1: 1, 000-5, 000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein BTC-beta. The membrane blot was probed with anti BTC-beta primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein BTC-beta from E.coli cell (20kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Betacellulin (BTC) is a member of the epidermal growth factor (EGF) family which binds and activates the EGF receptor and erbB-4, synthesized primarily as a transmembrane precursor.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat BTCbeta has not been tested yet.
References
- Mara Victoria G³mez-Gaviro, et al. Betacellulin promotes cell proliferation in the neural stem cellniche and stimulates neurogenesis. Proc Natl Acad Sci U S A. 2012 January 24; 109(4): 13171322.
- Bela Anand-Apte, et al. Betacellulin Induces Increased Retinal Vascular Permeability in Mice. PLoSOne. 2010; 5(10): e13444.
Expiration:
12 months from the date of shipment when stored properly.
