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Anti-bFGF / FGF basic / FGF2 Antibody, Biotinylated
Catalog #: DS-PB-00770
Rabbit Anti-Human bFGF / FGF basic / FGF2 [+Biotin]
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | This product is specific for human FGF basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues. FGF 2 is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis. |
| Host Species | Rabbit |
| Protein Name / Synonyms | bFGF / FGF basic / FGF2 |
| Preparation | Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
| Concentration (lot specific) | IgG concentration 50ug/ml |
| Recommended Applications | ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting |
| Reconstitution | Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Storage/Stability
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be storde undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
Recombinant human FGF basic.
Preservative:
None present.
Expiration:
12 months from the date of shipment when stored properly.