Mouse anti-bFGF/FGF basic/FGF2 Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | NM_002006 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was full recombinant protein derived from human bFGF. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by protein A/G affinity chromatography. |
| Recommended Applications | ELISA: By direct ELISA, at least 1:10, 000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431, Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application. |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, is members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (HST/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The anti-human bFGF antibody reacts with recombinant protein and cell lysate at various concentrations. Its cross reactivity with mouse, rat and other species is not tested.
References
- Miyamoto, M., et al. 1993. Molecular cloning of a novel cytokine cDNA encoding the ninth member of the fibroblast growth factor family, which has a unique secretion property. Mol. Cell. Biol. 13: 4251-4259.
- Beer, H.D., et al. 1997. Mouse fibroblast growth factor 10: cDNA cloning, protein characterization and regulation of mRNA expression. Oncogene 15: 2211-2218.
Expiration:
12 months from the date of shipment when stored properly.
