Rabbit Anti-Human Bi-Phospho-ERK1/2(T202/Y204) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P27361 |
| Gene Id | 5595 |
| Gene Symbols | MAPK3 |
| Protein Name / Synonyms | Bi-Phospho-ERK1/2(T202/Y204) |
| Clonality | Polyclonal |
| Preparation | This ERK1/2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T202/Y204 of human ERK1/2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, Dot Blotting |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Specificity
Predicted Reactivity
Mouse, Rat, Drosophila
Other Names
MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; Microtubule-associated protein 2 kinase; p44-ERK1
NCBI Accession #
NP_001035145.1;NP_001103361.1;NP_002737.2
Other Accession #
P21708; Q63844; P40417
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43136
Recommended Dilutions
DB: 1:500; WB: 1:1000
 | Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody (Cat# 102-15492) on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
 | Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) (Cat# 102-15492). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane). |
 | Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) (Cat# 102-15492). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane) . |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.