Anti-Bi-Phospho-Syk (Y525/526) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P43405
Gene Id	6850
Gene Symbols	SYK
Protein Name / Synonyms	Bi-Phospho-Syk(Y525/526)
Clonality	Polyclonal
Preparation	This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Specificity

Predicted Reactivity

Mouse, Rat, Pig

Other Names

SYK; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk

NCBI Accession #

NP_001128524.1;NP_001167638.1;NP_001167639.1;NP_003168.2

Other Accession #

Q64725; Q00655; P48025

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

72066

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-Phospho-Syk Pab(Cat# 102-15362) in mouse liver tissue lysate (35ug/lane). Phospho-Syk(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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