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Anti-BIM Antibody, Biotinylated
Catalog #: RB-02-0032B-50
Rabbit anti-BIM Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43521 |
| Gene Id | 10018 |
| Gene Symbols | BCL2L11 BIM |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide (BIM). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Immunogen Sequence | CERPPQLRPGAPTSLQTEPQG |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 32, 000), Western Blotting (recommended work dilution= 1: 1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains. Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1. Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated. Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody can specifically bind to human BIM. The cross reactivity with mouse and rat BIM was not tested.
References
- O'Connor L, et al. (1998) Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 17(2):384-95.
- Hsu, SY. et al. (1998) BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domaincontaining proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol Endocrinol.12(9):1432-40.
Expiration:
12 months from the date of shipment when stored properly.