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Anti-Bim Antibody, Biotinylated
Catalog #: RB-02-0035B-50
Rabbit anti-Bim Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43521 |
| Gene Id | 10018 |
| Gene Symbols | BCL2L11 BIM |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a synthetic peptide derived from human Tau. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells derived from the immunized mouse. The IgM fraction of cell culture supernatant was purified by affinity chromatography. |
| Immunogen Sequence | CAQELRRIGDEFNAYYARRLEK |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:2, 000-5, 000), Western Blotting( recommended work dilution= 5 µg/ml), Immunoprecipitation, Blocking peptide with pThr231 (20 µL ( µg/ml))(recommended work dilution= ( µg/ml) Determined by customer) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members, including Bad, Bid, Bik, Hrk and Noxa, which contain a BH3 domain but lack other conserved BH1 or BH2 domains. Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1. Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated. Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat Bim(GL) has not yet been tested.
Related Products
- Rabbit Anti-Human BIM (catalog #: RB-02-0032)
- Rabbit Anti-BIM (catalog #: DS-PB-02410)
References
- Sunters A, et al..(2003) FoxO3a transcriptional regulation of Bim controls apoptosis in paclitaxel-treated breast cancer cell lines. J Biol Chem. 278(50):49795-805.
- Dai DL, et al..(2008) Bim expression is reduced in human cutaneous melanomas. J Invest Dermatol. 128(2):403-7.
Expiration:
12 months from the date of shipment when stored properly.