Anti-BMI1 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P35226
Gene Id	648
Gene Symbols	BMI1
Protein Name / Synonyms	BMI1
Clonality	Monoclonal
Preparation	This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Specificity

Other Names

BMI1; PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51

NCBI Accession #

NP_001190991.1;NP_005171.4

Other Accession #

NP_005171.4

Format

Type

Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

36949

Recommended Dilutions

WB: 1:100-250; IF: 1:10-50

	BMI1 Antibody (Cat# 102-10158) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).
	Confocal immunofluorescent analysis of BMI1 Antibody (Cat# 102-10158) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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