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Anti-Bovine Granulysin Antibody, Biotinylated

Catalog #: RB-06-0001B-50

Rabbit anti-Bovine Granulysin Biotinylated Antibody, 50 ug

Product Description

Specifications

Size50 µg
Estimated Lead Time1-2 business days
SpeciesBovine (cow)
Host SpeciesRabbit
Accession Number
NP_001068611
ClonalityPolyclonal
Immunogen
Immunogen was synthetic peptide derived from bovine Granulysin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
Immunogen Sequence
CVDIKMCKSKPVGFI
IsotypeIgG
Recommended ApplicationsELISA (recommended work dilution= 1 : 64, 000)
Reconstitution
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Research AreaCardiovascular Disease
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Granulysin is also called as Lymphokine LAG-2, Protein NKG5 and T-cell activation protein 519.The protein has been found in many species. It is belonged to antimicrobial protein because it can kill intracellular pathogens, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Currently, one of the most interesting antimicrobial activities associated with granulysin is the ability to directly kill mycobacteria. In contrast to other antimicrobial peptides, such as defensins, granulysin is confined to the cytotoxic granules of NK cells and T lymphocytes. Bovine Granulysin, consisting of 146 amino acids is one of attractive anti-pathogen candidates.

Storage / Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Specificity

Rabbit anti-Bovine Granulysin antibody can specifically detect the synthetic peptide, but not unrelated peptides and proteins. Cross reactivity with other species has not yet been tested.

References

  • Krensky A.M. Granulysin: a novel antimicrobial peptide of cytolytic T lymphocytes and natural killer cells. Biochem. Pharmacol. 59:317-320(2000)
  • Stenger S., et.al., An antimicrobial activity of cytolytic T cells mediated by granulysin. Science 282:121- 125(1998); Janice J. Endsley, et.al., Characterization of Bovine Homologues of Granulysin and NK-lysin. The Journal of Immunology, 173: 2607-2614(2004).
Expiration:
12 months from the date of shipment when stored properly.