Synonyms
BPGM antibody, "erythrocyte 2,3-bisphosphoglycerate mutase antibody", "2,3-diphosphoglycerate mutase antibody", "2,3-bisphosphoglycerate synthase antibody", bisphosphoglycerate mutase antibody, BPG-dependent PGAM antibody, "2,3-bisphosphoglycerate mutase, erythrocyte antibody", "2,3-bisphosphoglycerate mutase antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
30 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 246 of BPGM (Uniprot ID# P07738)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: mouse spleen
12% SDS PAGE
107-10669 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BPGM(107-10669) antibody at 1:100 dilution.

Sample(30 ug whole cell lysate)
A:293T
B:A431
C:H1299
12% SDS PAGE
107-10669 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.