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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 9577 |
| Protein Name / Synonyms | BRCC45 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
BRCC4 antibody, BRCC45 antibody, BRE antibody, BRCA1/BRCA2-containing complex subunit 45 antibody, BRCA1/BRCA2-containing complex, subunit 4 antibody, BRCA1-A complex subunit BRE antibody, brain and reproductive organ-expressed protein antibody, brain and reproductive organ-expressed (TNFRSF1A modulator) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
44 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 9 and 328 of BRCC45
Applications
Applications Tested
ICC/IF,Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
BRE antibody immunoprecipitates BRE protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. Control with 2.5 µg of preimmune rabbit IgG B. Immunoprecipitation of BRE protein by 2.5 µg of BRE antibody (107-10526) 10% SDS-PAGE The immunoprecipitated BRE protein was detected by BRE antibody (107-10526) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
BRCC45 antibody detects BRE protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (107-10526) dilution: 1:1000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (107-10526) dilution: 1:1000
BRCC45 antibody detects BRE protein by Western blot analysis.
A. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (107-10526) dilution: 1:1000
A. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (107-10526) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE(107-10526) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
